Thermal and pressure inactivation of subtilisin BPN'
Makimoto Syoichi; Wada Aya; Taniguchi Yoshihiro
Abstract:The pressure effects on thermal inactivation of subtilisin BPN' (S-BPN' ) and its pressure inactivation have been studied by measuring the rate of hydrolysis of succinyl-l-alanyl-l-alanyl-l-prolyl-l-phenylalanine-p-nitroanili de (SAAPFpNA) catalyzed by S-BPN' in the temperature range of 15 - 67.5 degree and at the pressures up to 700 MPa. The apparent rate of hydrolysis decreased with time above a certain temperature and pressure. The decrease is due to the decrease in S-BPN' activity. The enthalpy change of the thermal inactivation was 100 - 220 kJ mol-1, and the volume change of this process was 18 - -4 cm3 mol-1. While S-BPN' was inactivated in the pressure range of 500 - 700 MPa at 15 - 45 degree, the corresponding volume change was -13 - -22 cm3 mol-1. Key Words:thermal inactivation, pressure inactivation, volume change, subtilisin BPN