Adsorption of Proteins on Needle-Like Heat-Treated Hydroxyapatite
Osamu TAKAGIi and Suguru SUZUKI
Abstract:An adsorption of proteins on the needle-like hydroxyapatite(N-Hap) after heat treatment at 100 - 700was investigated. Adsorbed amount of Bovine Serum Arubumine(BSA) as an acidic protein decreased with increasing heat treatment temperature except 700Cwhereas that for Lysozyme chloride(LYZ) as a basic protein increased with treatment temperature. Colloidal vibration potentials(CVP) of all N-Haps showed negative potentials in a phosphate buffer solution at pH6.8, and shifted to more negative regions with increasing treatment temperature. Based on the XRD patterns, the N-Haps treated at 100-400 were Hap phase only, but -TCP(CaR(POS)Q) phase appeared in the N-Hap treated at 700. Key Words:Hydroxyapatite, Colloidal vibration potential, Protein, Adsorption